Western blot analysis of TORC1 using anti-TORC1 antibody (PA1987). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TORC1 antigen affinity purified polyclonal antibody (Catalog # PA1987) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TORC1 at approximately 78KD. The expected band size for TORC1 is at 67KD.
Western blot analysis of TORC1 using anti-TORC1 antibody (PA1987). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TORC1 antigen affinity purified polyclonal antibody (Catalog # PA1987) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TORC1 at approximately 78KD. The expected band size for TORC1 is at 67KD.
Anti-TORC1/CRTC1 Antibody Picoband(r)
PA1987
ApplicationsWestern Blot
Product group Antibodies
ReactivityBovine, Human, Rat
TargetCRTC1
Overview
- SupplierBoster Bio
- Product NameAnti-TORC1 Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteWB: The detection limit for CRTC1 is approximately 1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot
- Applications SupplierWB
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID23373
- Target nameCRTC1
- Target descriptionCREB regulated transcription coactivator 1
- Target synonymsCREB-regulated transcription coactivator 1; Mam-2; MAML2; Mastermind-like protein 2; MECT1; mucoepidermoid carcinoma translocated protein 1; TORC1; TORC-1; transducer of regulated cAMP response element-binding protein (CREB) 1; WAMTP1
- HostRabbit
- IsotypeIgG
- Protein IDQ6UUV9
- Protein NameCREB-regulated transcription coactivator 1
- Scientific DescriptionBoster Bio Anti-TORC1/CRTC1 Antibody catalog # PA1987. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityBovine, Human, Rat
- Reactivity SupplierHuman, Rat, Bovine
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203