Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using TRAF6 Antibody(M00185) TRAF6 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TRAF6 Antibody (M00185)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using TRAF6 Antibody(M00185) TRAF6 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TRAF6 Antibody (M00185)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-TRAF6 Rabbit Monoclonal Antibody
M00185
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTRAF6
Overview
- SupplierBoster Bio
- Product NameAnti-TRAF6 Rabbit Monoclonal Antibody
- Delivery Days Customer9
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDBOG-20
- FormulationLiquid
- Gene ID7189
- Target nameTRAF6
- Target descriptionTNF receptor associated factor 6
- Target synonymsE3 ubiquitin-protein ligase TRAF6; interleukin-1 signal transducer; MGC:3310; RING finger protein 85; RING-type E3 ubiquitin transferase TRAF6; RNF85; TNF receptor-associated factor 6; TNF receptor-associated factor 6, E3 ubiquitin protein ligase
- HostRabbit
- IsotypeIgG
- Protein IDQ9Y4K3
- Protein NameTNF receptor-associated factor 6
- Scientific DescriptionBoster Bio Anti-TRAF6 Rabbit Monoclonal Antibody catalog # M00185. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C
- UNSPSC12352203