Anti-trans-sialidase [13G9]

Absolute Antibody

Anti-trans-sialidase [13G9]

7

13G9 can be used for IF with whole parasites which allows membrane localization of TS to be observed. The antibody can inhibit TS-mediated sialylation of the parasite via the transfer of sialic acid from the enivronment to the surface of the parasite. Cell invasion (a process in which TS plays a key role) is also inhibited by 13G9 and was shown to greatly reduce the number of T. cruzi-infected cells. Passive transfer of 13G9 to T. cruzi-infected mice provided protection against the delterious effects that TS has on the immune system and platelets. The 13G9 Fab fragment was also used to crystallize TS for structure studies and this allowed the binding to TS to be seen.

ImmunoFluorescence, Neutralisation/Blocking, Other Application

IF; neutralize; crystallization

Research Use Only

Monoclonal

13G9

Mouse

IgG2a

Trypanosoma cruzi

13G9 was prepared by immnuizing mice with recombinant TS (Δ1443TS), which is fully active but includes a deletion of a non-neutralizing epitope (speeds up production of neutralizing antibodies). The antibody was selected for by screening using a TS-inhibition assay.

-20°C,2°C to 8°C

12352203