Figure 1. Western blot analysis of TRAP1 using anti-TRAP1 antibody (PB9834). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug, Lane 2: Rat Kidney Tissue Lysate at 50ug, Lane 3: Rat Brain Tissue Lysate at 50ug, Lane 4: SMMC Whole Cell Lysate at 40ug, Lane 5: PANC Whole Cell Lysate at 40ug, Lane 6: A549 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAP1 antigen affinity purified polyclonal antibody (Catalog # PB9834) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAP1 at approximately 80 kDa. The expected band size for TRAP1 is at 80 kDa.
Figure 1. Western blot analysis of TRAP1 using anti-TRAP1 antibody (PB9834). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug, Lane 2: Rat Kidney Tissue Lysate at 50ug, Lane 3: Rat Brain Tissue Lysate at 50ug, Lane 4: SMMC Whole Cell Lysate at 40ug, Lane 5: PANC Whole Cell Lysate at 40ug, Lane 6: A549 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAP1 antigen affinity purified polyclonal antibody (Catalog # PB9834) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAP1 at approximately 80 kDa. The expected band size for TRAP1 is at 80 kDa.
Anti-TRAP1 Antibody Picoband(r)
PB9834
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetTRAP1
Overview
- SupplierBoster Bio
- Product NameAnti-TRAP1 Picoband Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID10131
- Target nameTRAP1
- Target descriptionTNF receptor associated protein 1
- Target synonymsheat shock protein 75 kDa, mitochondrial; HSP 75; HSP75; HSP90L; testicular tissue protein Li 209; TNFR-associated protein 1; TRAP-1; tumor necrosis factor type 1 receptor-associated protein
- HostRabbit
- IsotypeIgG
- Protein IDQ12931
- Protein NameHeat shock protein 75 kDa, mitochondrial
- Scientific DescriptionBoster Bio Anti-TRAP1 Antibody Picoband® catalog # PB9834. Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203