Figure 1. Western blot analysis of TRIM36 using anti-TRIM36 antibody (A10270-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIM36 antigen affinity purified polyclonal antibody (Catalog # A10270-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIM36 at approximately 83 kDa. The expected band size for TRIM36 is at 83 kDa.
Figure 1. Western blot analysis of TRIM36 using anti-TRIM36 antibody (A10270-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIM36 antigen affinity purified polyclonal antibody (Catalog # A10270-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIM36 at approximately 83 kDa. The expected band size for TRIM36 is at 83 kDa.
Anti-TRIM36 Antibody Picoband(r)
A10270-3
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTRIM36
Overview
- SupplierBoster Bio
- Product NameAnti-TRIM36 Antibody Picoband(r)
- Delivery Days Customer9
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID55521
- Target nameTRIM36
- Target descriptiontripartite motif containing 36
- Target synonymsANPH; ANPH1; E3 ubiquitin-protein ligase TRIM36; HAPRIN; RBCC728; RING finger protein 98; RING-type E3 ubiquitin transferase TRIM36; RNF98; tripartite motif protein 36; tripartite motif-containing protein 36; zinc-binding protein Rbcc728
- HostRabbit
- IsotypeIgG
- Protein IDQ9NQ86
- Protein NameE3 ubiquitin-protein ligase TRIM36
- Scientific DescriptionBoster Bio Anti-TRIM36 Antibody Picoband® catalog # A10270-3. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203