Figure 1. Western blot analysis of TRIM5 using anti-TRIM5 antibody (A01359-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A375 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIM5 antigen affinity purified polyclonal antibody (Catalog # A01359-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIM5 at approximately 56KD. The expected band size for TRIM5 is at 56KD.
Figure 1. Western blot analysis of TRIM5 using anti-TRIM5 antibody (A01359-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A375 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIM5 antigen affinity purified polyclonal antibody (Catalog # A01359-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIM5 at approximately 56KD. The expected band size for TRIM5 is at 56KD.
Anti-TRIM5 Antibody Picoband(r)
A01359-1-BIOTIN
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetTRIM5
Overview
- SupplierBoster Bio
- Product NameAnti-TRIM5 Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateBiotin
- Gene ID85363
- Target nameTRIM5
- Target descriptiontripartite motif containing 5
- Target synonymsring finger protein 88; RING-type E3 ubiquitin transferase TRIM5; RNF88; TRIM5alpha; tripartite motif containing 5 transcript variant iota; tripartite motif containing 5 transcript variant kappa; tripartite motif protein TRIM5; tripartite motif-containing protein 5
- HostRabbit
- IsotypeIgG
- Protein IDQ9C035
- Protein NameTripartite motif-containing protein 5
- Scientific DescriptionBoster Bio Anti-TRIM5 Antibody Picoband® catalog # A01359-1. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203