Anti-Tritrpticin [6C6D7]

Absolute Antibody

Anti-Tritrpticin [6C6D7]

9

This antibody binds tritrpticin (VRRFPWWWPFLRR), which is a member of the cathelicidin family, a 13mer tryptophan-rich group of diverse cationic antimicrobial peptides found in neutrophil granules. It has a broad spectrum activity against a range of micro

The binding characterization of this antibody was done using ELISA. In an indirect-binding ELISA where the peptides were directly immobilized on to the ELISA plate, mAb 6C6D7 could bind to tritrpticin and its N-terminal deleted antibacterial analogs at nanomolar concentrations. The binding affinity of this antibody to K-tritrpticin was analyzed using surface plasmon resonance and it was reported to be Kd= 350 nM. The binding of 6C6D7 to tritrpticin is characterized by negligible change in deltaGeq with respect to temperature that suggests that the antibody does not undergo any major conformational alteration during the binding of peptide antigen. This antibody was also used to bind tritripticin and related peptides to further analyze their anti-microbial activity (PMID: 24086578).

ELISA

Research Use Only

Monoclonal

6C6D7

Rabbit

IgG

Porcine

-20°C,2°C to 8°C

12352203