Figure 1. Western blot analysis of TRPM4 using anti-TRPM4 antibody (PB9902). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPM4 antigen affinity purified polyclonal antibody (Catalog # PB9902) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPM4 at approximately 167 kDa. The expected band size for TRPM4 is at 134 kDa.
Figure 1. Western blot analysis of TRPM4 using anti-TRPM4 antibody (PB9902). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPM4 antigen affinity purified polyclonal antibody (Catalog # PB9902) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPM4 at approximately 167 kDa. The expected band size for TRPM4 is at 134 kDa.
Anti-TRPM4 Antibody Picoband(r)
PB9902
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTRPM4
Overview
- SupplierBoster Bio
- Product NameAnti-TRPM4 Picoband Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID54795
- Target nameTRPM4
- Target descriptiontransient receptor potential cation channel subfamily M member 4
- Target synonymscalcium-activated non-selective cation channel 1; EKVP6; hTRPM4; long transient receptor potential channel 4; LTrpC4; melastatin-4; PFHB1B; transient receptor potential cation channel subfamily M member 4; TRPM4B
- HostRabbit
- IsotypeIgG
- Protein IDQ8TD43
- Protein NameTransient receptor potential cation channel subfamily M member 4
- Scientific DescriptionBoster Bio Anti-TRPM4 Antibody Picoband® catalog # PB9902. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203