Figure 1. Western blot analysis of TRPV5 using anti-TRPV5 antibody (PB9518). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Pancreas Tissue Lysate at 50ug, Lane 2: Rat Lung Tissue Lysate at 50ug, Lane 3: Rat Intestine Tissue Lysate at 50ug, Lane 4: SW620 Whole Cell Lysate at 40ug, Lane 5: COLO320 Whole Cell Lysate at 40ug, Lane 6: 293T Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPV5 antigen affinity purified polyclonal antibody (Catalog # PB9518) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPV5 at approximately 83 kDa. The expected band size for TRPV5 is at 83 kDa.
Figure 1. Western blot analysis of TRPV5 using anti-TRPV5 antibody (PB9518). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Pancreas Tissue Lysate at 50ug, Lane 2: Rat Lung Tissue Lysate at 50ug, Lane 3: Rat Intestine Tissue Lysate at 50ug, Lane 4: SW620 Whole Cell Lysate at 40ug, Lane 5: COLO320 Whole Cell Lysate at 40ug, Lane 6: 293T Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPV5 antigen affinity purified polyclonal antibody (Catalog # PB9518) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPV5 at approximately 83 kDa. The expected band size for TRPV5 is at 83 kDa.
Anti-TRPV5 Antibody Picoband(r)
PB9518
ApplicationsWestern Blot
Product group Antibodies
ReactivityHamster, Human, Rat
TargetTRPV5
Overview
- SupplierBoster Bio
- Product NameAnti-TRPV5 Picoband Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID56302
- Target nameTRPV5
- Target descriptiontransient receptor potential cation channel subfamily V member 5
- Target synonymscalcium transport protein 2; calcium transporter 2; CAT2; ECAC1; epithelial calcium channel 1; osm-9-like TRP channel 3; OTRPC3; transient receptor potential cation channel subfamily V member 5
- HostRabbit
- IsotypeIgG
- Protein IDQ9NQA5
- Protein NameTransient receptor potential cation channel subfamily V member 5
- Scientific DescriptionBoster Bio Anti-TRPV5 Antibody Picoband® catalog # PB9518. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHamster, Human, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203