Figure 1. Western blot analysis of TSG101 using anti-TSG101 antibody (M01233). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human COLO320 whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSG101 antigen affinity purified monoclonal antibody (Catalog # M01233) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TSG101 at approximately 44 kDa. The expected band size for TSG101 is at 44 kDa.