Figure 1. Western blot analysis of TSNAXIP1 using anti-TSNAXIP1 antibody (A18904). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: rat testis tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSNAXIP1 antigen affinity purified polyclonal antibody (Catalog # A18904) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TSNAXIP1 at approximately 70 kDa, 50 kDa. The expected band size for TSNAXIP1 is at 77 kDa.
Figure 1. Western blot analysis of TSNAXIP1 using anti-TSNAXIP1 antibody (A18904). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: rat testis tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSNAXIP1 antigen affinity purified polyclonal antibody (Catalog # A18904) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TSNAXIP1 at approximately 70 kDa, 50 kDa. The expected band size for TSNAXIP1 is at 77 kDa.
Anti-TSNAXIP1 Antibody Picoband(r)
A18904-DYLIGHT550
TargetTSNAXIP1
Product group Antibodies
Overview
- SupplierBoster Bio
- Product NameAnti-TSNAXIP1 Antibody Picoband(r)
- Delivery Days Customer9
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- Concentration500 ug/ml
- Gene ID55815
- Target nameTSNAXIP1
- Target descriptiontranslin associated factor X interacting protein 1
- Target synonymstranslin-associated factor X-interacting protein 1; trax-interacting protein 1; TXI1
- Protein IDQ2TAA8
- Protein NameTranslin-associated factor X-interacting protein 1
- Scientific DescriptionBoster Bio Anti-TSNAXIP1 Antibody Picoband® catalog # A18904. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203