Immunohistochemical analysis of paraffin-embedded human thyroid, using TTF1 Antibody(M01322-1) NKX2-1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-NKX2-1 Antibody (M01322-1)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-TTF1 NKX2-1 Rabbit Monoclonal Antibody
M01322-1
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNKX2-1
Overview
- SupplierBoster Bio
- Product NameAnti-TTF1 Rabbit Monoclonal Antibody
- Delivery Days Customer9
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDBIG-14
- FormulationLiquid
- Gene ID7080
- Target nameNKX2-1
- Target descriptionNK2 homeobox 1
- Target synonymsBCH; BHC; homeobox protein NK-2 homolog A; homeobox protein Nkx-2.1; NK-2; NK-2 homolog A; NKX2.1; NKX2A; NMTC1; T/EBP; TEBP; thyroid nuclear factor 1; thyroid transcription factor 1; thyroid-specific enhancer-binding protein; TITF1; TTF1; TTF-1
- HostRabbit
- IsotypeIgG
- Protein IDP43699
- Protein NameHomeobox protein Nkx-2.1
- Scientific DescriptionBoster Bio Anti-TTF1 NKX2-1 Rabbit Monoclonal Antibody catalog # M01322-1. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C
- UNSPSC12352203