
Figure 1. Western blot analysis of TUBGCP3 using anti-TUBGCP3 antibody (A10799-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TUBGCP3 antigen affinity purified polyclonal antibody (Catalog # A10799-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TUBGCP3 at approximately 104 kDa. The expected band size for TUBGCP3 is at 104 kDa.
Anti-TUBGCP3 Antibody Picoband(r)
A10799-2-BIOTIN
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetTUBGCP3
Overview
- SupplierBoster Bio
- Product NameAnti-TUBGCP3 Antibody Picoband(r)
- Delivery Days Customer9
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateBiotin
- Gene ID10426
- Target nameTUBGCP3
- Target descriptiontubulin gamma complex associated protein 3
- Target synonyms104p; ALP6; gamma-ring complex protein 104 kDa; gamma-tubulin complex component 3; GCP3; Grip104; SPBC98; Spc98; Spc98p; spindle pole body protein Spc98 homolog
- HostRabbit
- IsotypeIgG
- Protein IDQ96CW5
- Protein NameGamma-tubulin complex component 3
- Scientific DescriptionBoster Bio Anti-TUBGCP3 Antibody Picoband® catalog # A10799-2. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203