Anti-TWEAK [MTW-1]

Absolute Antibody

Anti-TWEAK [MTW-1]

9

This antibody is specific for murine TWEAK. It has been shown not to react with TNF-alpha, FasL, and TRAIL.

The specificity of this antibody has been confirmed in flow cytometric analysis, where it reacts specifically with mTWEAK-transfected L5178Y cells, and not with parental L5178Y cells or cells transfected with related TNF family members (Nakayama et al, 2003). Additionally, this antibody has been used to assess TWEAK cell surface expression in flow cytometric analysis (Nakayama et al, 2003). This antibody has been shown to block CD8-mTWEAK cytotoxicity against L5178Y cells over-expressing murine Fn14, the TWEAK receptor (Nakayama et al, 2003). When administered to collagen-induced arthritis (CIA) mice, this blocking antibody has been shown to have anti-inflammatory and antiangiogenic effects, with associated improvements to both the clinical and histological manifestations of CIA (Kamata et al, 2006). Additionally, administration of this antibody to denervated mice attenuates denervation-induced skeletal muscle loss (Mittal et al, 2010). Finally, administration of this antibody to CD163-/- mice subject to hind limb ischaemia inhibits the increased perfusion resulting from CD163 deficiency (Akahori et al, 2015).

Flow Cytometry, Neutralisation/Blocking

Research Use Only

Monoclonal

MTW-1

Tnfsf12

tumor necrosis factor (ligand) superfamily, member 12

A; Apo3l; DR; Dr3l; Dr3lg; TNF-related weak inducer of apoptosis; tumor necrosis factor ligand superfamily member 12; Twea; Tweak

Mouse

IgG2a

Tumor necrosis factor ligand superfamily member 12

This chimeric mouse antibody was made using a variable domain sequence of the original rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Mouse

-20°C,2°C to 8°C

12352203