Anti-VACV [SC212]

Absolute Antibody

Anti-VACV [SC212]

7

The antibody is specific for VACV.

The scFv fragment was converted to human IgG1 (VC212). The binding affinity of VC212 was estimated by competition ELISA to IC50 values 22 microg/ml (146.67 nM). The humanized version of the antibody neutralized the VACV, as evidenced by the plaque reduction neutralization test result with a PRNT50 of ~0.16 mg/ml (~1.07 microM) (Shin et al., 2019; PMID: 30856707).

ELISA, Neutralisation/Blocking

ELISA; in vitro neutralization

Research Use Only

Monoclonal

SC212

Rabbit

IgG

This full-length chimeric rabbit antibody was made using the variable domain sequences of the original Human scFv format for improved compatibility with existing reagents assays and techniques.

Virus

Vaccinia virus

A phage display library of human single chain variable fragment (scFv) antibodies was constructed from the B cells of healthy volunteers boosted by the VACV vaccine. Human scFv was isolated by panning against VACV.

-20°C,2°C to 8°C

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