Anti-VAP-1 [TK8-14]

Absolute Antibody

Anti-VAP-1 [TK8-14]

9

This antibody is specific for human VAP-1, a glycosylated homodimeric membrane protein consisting of two 90 kDa subunits connected by disulfide bonds. The recognized epitope is present both in dimeric and monomeric forms of VAP-1.

The original version of this antibody (mouse IgG2a) was generated, and its specificity was characterized by reciprocal immunoprecipitation with another anti-VAP-1 antibody (1B2). In gel analyses, this antibody was shown to react with the dimeric 170-kDa VAP-1 and, under reducing conditions, with the monomeric 90-kDa form of VAP-1. Furthermore, it was used for both immunofluorescence staining and flow cytometry analysis to assess the expression of VAP-1 in rat HEV-like endothelial cell line Ax transfectants (Salmi et al., 1998; PMID: 9605169). The original version of this antibody was biotinylated and characterized via various assays. In an ELISA, it was used to detect serum VAP-1 levels. In a Western blot, it was employed to analyze VAP-1 presence and molecular weight. In immunoaffinity chromatography, it was used to purify VAP-1 from serum samples. In an adhesion assay, it was used to specifically deplete sVAP-1 from serum (Kurkijaervi et al., 1998; PMID: 9686623). This antibody was used in transwell migration assays to study the phenotype of transmigrated monocytes by flow cytometry; it was employed to pretreat primary human hepatic sinusoidal endothelial cells to study its effect on monocyte transmigration. The results showed a significant reduction in monocyte transmigration upon pretreatment, indicating the antibodys role as an inhibitor of VAP-1 function (Zimmermann et al., 2012; DOI: 10.1136/gutjnl-2012-302514b.126). This antibody was used in flow cytometry and immunocytochemistry to detect and characterize human hematopoietic stem cells based on VAP-1 expression. In flow cytometry, this antibody was utilized to stain fresh human bone marrow and cord blood (CB) cells. In an immunocytochemistry assay, it was used to stain FACS-sorted CD34+ CB cells for visualization under a microscope. Furthermore, TK8-14 was utilized in RNAseq analysis to investigate gene expression profiles of VAP-1+ and VAP-1- HSCs (Iftakhar-E-Khuda et al., 2021; PMID: 34719737).

Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, Neutralisation/Blocking, Other Application

Research Use Only

Monoclonal

TK8-14

AOC3

amine oxidase copper containing 3

amine oxidase, copper containing 3 (vascular adhesion protein 1); copper amine oxidase; HPAO; membrane primary amine oxidase; placenta copper monamine oxidase; semicarbazide-sensitive amine oxidase; SSAO; VAP1; VAP-1

Mouse

IgG2a

Membrane primary amine oxidase

Human

-20°C,2°C to 8°C

12352203