Anti-VAP-1 [TK8-14]

Absolute Antibody

Anti-VAP-1 [TK8-14]

9

This antibody is specific for human VAP-1, a glycosylated homodimeric membrane protein consisting of two 90 kDa subunits connected by disulfide bonds. The recognized epitope is present both in dimeric and monomeric forms of VAP-1.

The original version of this antibody (mouse IgG2a) was generated, and its specificity was characterized by reciprocal immunoprecipitation with another anti-VAP-1 antibody (1B2). In gel analyses, this antibody was shown to react with the dimeric 170-kDa VAP-1 and, under reducing conditions, with the monomeric 90-kDa form of VAP-1. Furthermore, it was used for both immunofluorescence staining and flow cytometry analysis to assess the expression of VAP-1 in rat HEV-like endothelial cell line Ax transfectants (Salmi et al., 1998; PMID: 9605169). The original version of this antibody was biotinylated and characterized via various assays. In an ELISA, it was used to detect serum VAP-1 levels. In a Western blot, it was employed to analyze VAP-1 presence and molecular weight. In immunoaffinity chromatography, it was used to purify VAP-1 from serum samples. In an adhesion assay, it was used to specifically deplete sVAP-1 from serum (Kurkijaervi et al., 1998; PMID: 9686623). This antibody was used in transwell migration assays to study the phenotype of transmigrated monocytes by flow cytometry; it was employed to pretreat primary human hepatic sinusoidal endothelial cells to study its effect on monocyte transmigration. The results showed a significant reduction in monocyte transmigration upon pretreatment, indicating the antibodys role as an inhibitor of VAP-1 function (Zimmermann et al., 2012; DOI: 10.1136/gutjnl-2012-302514b.126). This antibody was used in flow cytometry and immunocytochemistry to detect and characterize human hematopoietic stem cells based on VAP-1 expression. In flow cytometry, this antibody was utilized to stain fresh human bone marrow and cord blood (CB) cells. In an immunocytochemistry assay, it was used to stain FACS-sorted CD34+ CB cells for visualization under a microscope. Furthermore, TK8-14 was utilized in RNAseq analysis to investigate gene expression profiles of VAP-1+ and VAP-1- HSCs (Iftakhar-E-Khuda et al., 2021; PMID: 34719737).

Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, Neutralisation/Blocking, Other Application

Research Use Only

Monoclonal

TK8-14

AOC3

amine oxidase copper containing 3

amine oxidase, copper containing 3 (vascular adhesion protein 1); copper amine oxidase; HPAO; membrane primary amine oxidase; placenta copper monamine oxidase; semicarbazide-sensitive amine oxidase; SSAO; VAP1; VAP-1

Rabbit

IgG

Membrane primary amine oxidase

This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Human

-20°C,2°C to 8°C

12352203