Anti-VCAM-1 [6C7.1]

Absolute Antibody

Anti-VCAM-1 [6C7.1]

7

This antibody is specific for murine VCAM-1. VCAM-1 is an important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and si

The original rat version of this antibody was used to treat experimental autoimmune encephalomyelitis (EAE in female SJL/N mice). It resulted in a later onset of tEAE (Engelhardt et al, 1998; pmid:9854045). To asses alpha4-integrin-mediated T cell interaction with VCAM-1 on blood-brain barrier-endothelium in experimental autoimmune encephalomyelitis flow cytometry was preformed on T cell blasts labeled with the rat version of this antibody (Vajkoczy et al, 2001; pmid:11518729). Futhermore, PLP-specific T lymphoblasts were used for immunofluorescence with the rat version of this antibody (Vajkoczy et al, 2001; pmid:11518729). And finally, mice were injected with the rat version of this antibody to asses how it can slow down progression of EAE. It showed an ability to slow EAE down (Vajkoczy et al, 2001; pmid:11518729). In research on EAE, T line cells were incubated with the rat version of this antibody. This was then used for flow cytometry (Laschinger and Engelhardt, 200; pmid:10626664). To evaluate vascular luminal VCAM-1 expression in NOD and Blab/c mice the rat version of this antibody was injected in mice after which they were killed and the tissue analyzed (Xu et al, 2003; pmid:12756264). VCAM-1 expression in the dermal and pial microvasculatures was quantified using immunofluorescence with the rat version of this antibody (Norman et al, 2008; pmid:18426970). To investigate the influence of VCAM-1 on tumor progression, the rat version of this antibody was used for blocking of VCAM-1 (Dieterich et al, 2019; pmid:31344266). The rat version of this antibody was also utilized for immunofluorescence on mouse retinal microvascular endothelial cells (Liu et al, 2017; pmid:28931763).

Flow Cytometry, ImmunoFluorescence, ImmunoHistoChemistry, Neutralisation/Blocking

Therapeutic; IF; FC; IHC; blocking

Research Use Only

Monoclonal

6C7.1

Rat

IgG1

Vascular cell adhesion protein 1

Mouse

Mouse

This antibody was rasied against VCAM-1.

-20°C,2°C to 8°C

12352203