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IHC analysis of VCAM1 using anti-VCAM1 antibody (A01199). VCAM1 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-VCAM1 Antibody (A01199) overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
IHC analysis of VCAM1 using anti-VCAM1 antibody (A01199). VCAM1 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-VCAM1 Antibody (A01199) overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
IHC analysis of VCAM1 using anti-VCAM1 antibody (A01199). VCAM1 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-VCAM1 Antibody (A01199) overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Anti-VCAM1 Antibody Picoband(r)

Research Use Only
A01199
Boster Bio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityMouse, Rat
TargetVcam1
100 ug
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-VCAM1 Antibody
  • Delivery Days Customer
    9
  • Antibody Specificity
    No cross reactivity with other proteins.
  • Application Supplier Note
    Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Formulation
    Lyophilized
  • Gene ID22329
  • Target name
    Vcam1
  • Target description
    vascular cell adhesion molecule 1
  • Target synonyms
    CD106; vascular cell adhesion protein 1; Vcam; V-CAM 1; Vcam-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP29533
  • Protein Name
    Vascular cell adhesion protein 1
  • Scientific Description
    Boster Bio Anti-VCAM1 Antibody Picoband® catalog # A01199. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
  • Reactivity
    Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Effects of artesunate and ursolic acid on hyperlipidemia and its complications in rabbit. Wang YL et al., 2013 Nov 20, Eur J Pharm Sci
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