Anti-VEGF [G6-31]

Absolute Antibody

Anti-VEGF [G6-31]

9

G6-31 recognises a VEGF epitope which is conserved between human and mouse VEGF and overlaps with the receptor binding surface. The KD of G6-31 binding to m-VEGF and h-VEGF is ~0.9 and 1.5 nM respectively. Alanine-scanning mutagenesis of solvent-accessibl

A VEGF phage ELISA was used to measure binding affinity of G6-31 to individual alanine-substituted VEGF mutants versus wild type VEGF. Crystals of G6-31 and the receptor binding fragment of human VEGF have been solved at 3.6 A (Fuh et al, 2005). Treatment of mice undergoing E2-induced PRLoma with G6-31 was used to block VEGF-A, causing reductions in the Ki-67-positive anterior cells, increases in TUNEL-positive anerior cells, and repair of the microvessel count by CD34-immunohistochemistry (Miyajima et al, 2010). Fluorescence quenching solution assay and SPR have been used to determine Kon and Koff at 25 degrees celsius (Lee et al, 2004).

ImmunoFluorescence, ELISA, Neutralisation/Blocking, Other Application

Research Use Only

Monoclonal

G6-31

Vegfa

vascular endothelial growth factor A

V; vascular endothelial growth factor A; vascular permeability factor; Veg; Vegf; VEGF12; VEGF16; VEGF18; Vpf

Mouse

IgG2a

Vascular endothelial growth factor A

This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Mouse

-20°C,2°C to 8°C

12352203