Western blot analysis of VEGF Receptor 2 using anti-VEGF Receptor 2 antibody (A00901-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGF Receptor 2 antigen affinity purified polyclonal antibody (Catalog # A00901-2) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VEGF Receptor 2 at approximately 180KD. The expected band size for VEGF Receptor 2 is at 152KD.
Western blot analysis of VEGF Receptor 2 using anti-VEGF Receptor 2 antibody (A00901-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGF Receptor 2 antigen affinity purified polyclonal antibody (Catalog # A00901-2) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VEGF Receptor 2 at approximately 180KD. The expected band size for VEGF Receptor 2 is at 152KD.
Anti-VEGF Receptor 2/KDR Antibody Picoband(r)
A00901-2
ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetKDR
Overview
- SupplierBoster Bio
- Product NameAnti-VEGF Receptor 2 Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID3791
- Target nameKDR
- Target descriptionkinase insert domain receptor
- Target synonymsCD309; fetal liver kinase-1; FLK1; kinase insert domain receptor (a type III receptor tyrosine kinase); protein-tyrosine kinase receptor Flk-1; soluble VEGFR2; tyrosine kinase growth factor receptor; vascular endothelial growth factor receptor 2; VEGFR; VEGFR2
- HostRabbit
- IsotypeIgG
- Protein IDP35968
- Protein NameVascular endothelial growth factor receptor 2
- Scientific DescriptionBoster Bio Anti-VEGF Receptor 2/KDR Antibody Picoband® catalog # A00901-2. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203