Figure 1. Western blot analysis of VEGFR-1/FLT1 using anti-VEGFR-1/FLT1 antibody (A00534-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGFR-1/FLT1 antigen affinity purified polyclonal antibody (Catalog # A00534-5) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VEGFR-1/FLT1 at approximately 200 kDa. The expected band size for VEGFR-1/FLT1 is at 151 kDa.
Figure 1. Western blot analysis of VEGFR-1/FLT1 using anti-VEGFR-1/FLT1 antibody (A00534-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGFR-1/FLT1 antigen affinity purified polyclonal antibody (Catalog # A00534-5) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VEGFR-1/FLT1 at approximately 200 kDa. The expected band size for VEGFR-1/FLT1 is at 151 kDa.
Anti-VEGFR-1/FLT1 Antibody Picoband(r)
A00534-5
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetFLT1
Overview
- SupplierBoster Bio
- Product NameAnti-VEGFR-1/FLT1 Antibody Picoband(r)
- Delivery Days Customer9
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID2321
- Target nameFLT1
- Target descriptionfms related receptor tyrosine kinase 1
- Target synonymsFLT; FLT-1; fms related tyrosine kinase 1; fms-like tyrosine kinase 1; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor); tyrosine-protein kinase FRT; tyrosine-protein kinase receptor FLT; vascular endothelial growth factor receptor 1; vascular permeability factor receptor; VEGFR1; VEGFR-1
- HostRabbit
- IsotypeIgG
- Protein IDP17948
- Protein NameVascular endothelial growth factor receptor 1
- Scientific DescriptionBoster Bio Anti-VEGFR-1/FLT1 Antibody Picoband® catalog # A00534-5. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203