IHC analysis of Vinculin using anti-Vinculin antibody (MA1103). Vinculin was detected in frozen section of human placenta tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Vinculin Antibody (MA1103) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
IHC analysis of Vinculin using anti-Vinculin antibody (MA1103). Vinculin was detected in frozen section of human placenta tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Vinculin Antibody (MA1103) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Anti-Vinculin VCL Antibody (Monoclonal, hVIN-1)
MA1103
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
ReactivityBovine, Chicken, Human, Monkey, Mouse, Rabbit, Rat
TargetVCL
Overview
- SupplierBoster Bio
- Product NameAnti-Vinculin Antibody (Monoclonal, hVIN-1)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteOther applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
- Applications SupplierIHF, WB, IHC
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDhVIN-1
- Concentration100 ug/ml
- FormulationLyophilized
- Gene ID7414
- Target nameVCL
- Target descriptionvinculin
- Target synonymsCMD1W; CMH15; epididymis luminal protein 114; epididymis secretory sperm binding protein; HEL114; metavinculin; meta-vinculin; MV; MVCL; vinculin; vinculin isoform meta-VCL
- HostMouse
- IsotypeIgG1
- Protein IDP85972
- Protein NameVinculin
- Scientific DescriptionBoster Bio Anti-Vinculin VCL Antibody (Monoclonal, hVIN-1) catalog # MA1103. Tested in IF, IHC-F, ICC, WB applications. This antibody reacts with Chicken, Human, Monkey, Mouse, Rat.
- ReactivityBovine, Chicken, Human, Monkey, Mouse, Rabbit, Rat
- Reactivity SupplierChicken, Human, Mouse, Rat, Bovine, Rabbit
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203
References
- LRP6 promotes invasion and metastasis of colorectal cancer through cytoskeleton dynamics. Yao Q et al., 2017 Dec 12, OncotargetRead more