Anti-von Willebrand factor [82D6A3]

Absolute Antibody

Anti-von Willebrand factor [82D6A3]

9

This antibody is specific for human vWF, A3 domain.

This antibody was used as an inhibitor to study platelet adhesion to collagen VI and ECM. It was found to inhibit the binding of von Willebrand factor (VWF) to collagen I and III (Hoylaerts et al., 1997; PMID: 9164855). This antibody was found to have a comparable affinity for baboon vWF as for human vWF and inhibited the binding of baboon vWF to collagen with an inhibitory concentration of 50% (IC50) of 3.5 microg/ml. Furthermore, it prevented the binding of VWF to collagen and removed bound VWF from a collagen surface, albeit with a lower efficacy. Moreover, the antithrombotic efficacy of the antibody was evaluated in a modified Folts model in baboons; this antibody was used to inhibit the interaction between collagen and vWF to prevent arterial thrombosis in baboons (Wu et al., 2002; PMID: 11986216). The crystal structure of the antibodys Fab fragment, in complex with the vWF A3 domain, was determined (Staelens et al., 2006; PMID: 16314412).

Neutralisation/Blocking, Other Application

Research Use Only

Monoclonal

82D6A3

VWF

von Willebrand factor

coagulation factor VIII VWF; F8VWF; von Willebrand factor; VWD

Rabbit

IgG

von Willebrand factor

Human, Primate

-20°C,2°C to 8°C

12352203