Figure 1. Western blot analysis of WRN using anti-WRN antibody (PB10107). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WRN antigen affinity purified polyclonal antibody (Catalog # PB10107) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WRN at approximately 162kDa, 200 kDa. The expected band size for WRN is at 162 kDa.
Figure 1. Western blot analysis of WRN using anti-WRN antibody (PB10107). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WRN antigen affinity purified polyclonal antibody (Catalog # PB10107) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WRN at approximately 162kDa, 200 kDa. The expected band size for WRN is at 162 kDa.
Anti-Werners syndrome helicase WRN/WRN Antibody Picoband(r)
PB10107
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Rat
TargetWRN
Overview
- SupplierBoster Bio
- Product NameAnti-WRN Picoband Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID7486
- Target nameWRN
- Target descriptionWRN RecQ like helicase
- Target synonymsDNA helicase, RecQ-like type 3; exonuclease WRN; recQ protein-like 2; RECQ3; RECQL2; RECQL3; Werner syndrome ATP-dependent helicase; Werner syndrome RecQ like helicase; Werner syndrome, RecQ helicase-like
- HostRabbit
- IsotypeIgG
- Protein IDQ14191
- Protein NameWerner syndrome ATP-dependent helicase
- Scientific DescriptionBoster Bio Anti-Werners syndrome helicase WRN/WRN Antibody Picoband® catalog # PB10107. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203