Anti-XCR1 [5G7]

Absolute Antibody

Anti-XCR1 [5G7]

7

This antibody binds human XCR1, more particularly it strongly binds amino acids PESTTFFYYDLQ of the human XCR1. XRC1 is a receptor for chemokines SCYC1 and SCYC2 and expressed on 80% of CD8+ the dendritic cells. It also acts as a receptor for XCL1/Lymphot

This antibody is capable of binding human XCR1-EGFP-expressing B300.19 cells in a flow cytometry analysis. The determination of the binding epitope of this antibody was done using ELISA (US9371389). This antibody exhibits a significant effect in treating rheumatoid arthritis in the experiment of delayed-type hypersensitivity reaction induced by Mycobacterium butyricum delayed-type hypersensitivity reaction. The neutralizing activity of this antibody against human lymphotactin-induced migration of human XCR1-expressing cells was evaluated by in vitro chemotaxis assay. It was reported that this antibody completely inhibited cell migration at a concentration of about 3 microg/mL (US9371389). The effect of this antibody on ear swelling of DNFB (Dinitrofluorobenzene)-sensitized mice was done and it was reported that this antibody caused significant suppression of ear swelling 24 after induction. This antibody was also reported to show showed a very low reactivity to human CX3CR1-EGFP-expressing B300.19 cells (US9371389). The original antibody with saporin-conjugated secondary antibody showed growth suppression of human XCR1-EGFP-expressing B300.19 cells. The humanized version of this antibody is also available on request (US9371389).

Flow Cytometry, ELISA

ELISA; FC

Research Use Only

Monoclonal

5G7

XCR1

X-C motif chemokine receptor 1

CCXCR1; chemokine (C motif) receptor 1; chemokine (C motif) XC receptor 1; chemokine XC receptor 1; G protein-coupled receptor 5; GPR5; lymphotactin receptor; XC chemokine receptor 1

Rabbit

IgG

Chemokine XC receptor 1

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format for improved compatibility with existing reagents assays and techniques.

Human

Human

The original antibody was generated by immunizing XCR1 knockout mice with human XCR1-expressing B300.19 cells mixed with equal volume of GERBU adjuvant.

-20°C,2°C to 8°C

12352203