Anti-YFV-E protein [7A]

Absolute Antibody

Anti-YFV-E protein [7A]

7

The original antibody showed 50% and 100% neutralizing activity in plaque reduction neutralization assay (PRNT) against YFV strains 17D-204-WHO and Asibi at concentrations of approximately 1 microg/ml and 10 microg/ml respectively. The original antibody neutralized wild-type YFV strains of genotypes West Africa I (Nigeria 1987) and II (Asibi) and East/Central Africa (CAR 1986, Ethiopia 1961) with comparable efficiency in a PRNT on PS cells. In a competition ELISA using biotinylated scFv-7A, this antibody fragment competed for binding to YFV-17D virions with the other neutralizing antibody fragments. The original scFv version of this antibody was able to immunoprecipitate a 55-kDa protein, corresponding to the molecular weight of the envelope glycoprotein of YFV-17D virions (PMID: 15919103).

Functional Assay, ImmunoPrecipitation, ELISA, Neutralisation/Blocking

ELISA; IP; neutralize; functional assay

Research Use Only

Genome polyprotein

This full-length chimeric rabbit antibody was made using the variable domain sequences of the original Mouse scFv format for improved compatibility with existing reagents assays and techniques.

-20°C,2°C to 8°C

12352203