Anti-ZIKV E domain III [SMZAb1]

Absolute Antibody

Anti-ZIKV E domain III [SMZAb1]

7

SMZAb1 binds to domain III of the ZIKV E protein.

ELISA was used to confirm SMZAb1 binds to the ZIKV E domain, and neutralisation assays identified the IC50 of SMZAb1 as 5.4 ng/ml. Four macaques were injected with a cocktail of anti-Zika antibodies (SMZAb1, SMZAb2, SMZAb5) and then infected with ZIKV virus. The antibody cocktail appeared to confer sterilising immunity on the treated macaques and completely prevented viral replication, which was not seen with the control group (Magnani et al, 2017).

ELISA, Neutralisation/Blocking

ELISA; therapeutic; neutralisation assays

Research Use Only

Monoclonal

SMZAb1

Human

IgG1

Envelope protein

This reformatted human antibody was made using the variable domain sequences of the original Human IgG format, for improved compatibility with existing reagents, assays and techniques.

Virus

Zika virus

Antibody genes were cloned from blood-derived plasmablasts of a ZIKV-infected human subject from Colombia in the acute phase. Amplified heavy and light chains were tested for neutralisation, and SMZAb1 was selected from this.

-20°C,2°C to 8°C

12352203