Anti-ZP2 [IE3]

Absolute Antibody

Anti-ZP2 [IE3]

7

IE3 reacts specifically with ZP2 (East & Dean, 1984), where the specific epitope (TIKVVGGYQVNIRVGDTTTDVRYKDDMYHFFC) is at the N-terminus. ZP2 is a glycoprotein which forms the zona pellucida, an extracellular matrix surrounding mammalian oocytes. These gl

IE3 was shown to react with ZP2 by immunoprecipitation and immunofluorescence staining of various mouse tissue sections (East & Dean, 1984; Avella et al, 2014). IE3 was also shown to neutralise ZP2 activity in vivo by causing transient infertility in female mice.

ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, Neutralisation/Blocking

WB; IP; IF; IHC; NTRL

Research Use Only

Monoclonal

IE3

Zp2

zona pellucida glycoprotein 2

zona pellucida glycoprotein ZP2; zona pellucida protein A; zona pellucida sperm-binding protein 2; Zp-; Zp-2

Mouse

IgG2a

Zona pellucida sperm-binding protein 2

This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Mouse

Mouse

Osbourne-Mendel rats were immunised with solubilised zonae pellucidae from DBA/2 mice. Splenocytes were obtained from immunised rats and fused with the SP2/2 murine myeloma cell line to generate hybridomas.

-20°C,2°C to 8°C

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