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Western blot analysis of protein acetylation with anti-alpha-Tubulin (acetylated), mAb (TEU318) (Prod. No. AG-20B-0068).Method: HEK-293T cells grown in standard culture conditions, transfected with plasmids expressing the tubulin acetyl tra
Western blot analysis of protein acetylation with anti-alpha-Tubulin (acetylated), mAb (TEU318) (Prod. No. AG-20B-0068).Method: HEK-293T cells grown in standard culture conditions, transfected with plasmids expressing the tubulin acetyl tra
Western blot analysis of protein acetylation with anti-alpha-Tubulin (acetylated), mAb (TEU318) (Prod. No. AG-20B-0068).Method: HEK-293T cells grown in standard culture conditions, transfected with plasmids expressing the tubulin acetyl tra

anti-alpha-Tubulin (acetylated), mAb (TEU318)

Research Use Only
AG-20B-0068
AdipoGen Life Sciences
ApplicationsWestern Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityAll Species
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Overview

  • Supplier
    AdipoGen Life Sciences
  • Product Name
    anti-alpha-Tubulin (acetylated), mAb (TEU318)
  • Delivery Days Customer
    10
  • Antibody Specificity
    Detects K40 acetylation of alpha-tubulin; signal specifically increased by modification with tubulin acetyl transferase alpha-TAT1.
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    TEU318
  • Concentration
    1 mg/ml
  • Estimated Purity
    >95%
  • Formulation
    Liquid
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    Microtubules are key elements of the eukaryotic cytoskeleton that dynamically assemble from heterodimers of alpha- and beta-tubulin. Two different mechanisms can generate microtubule diversity: the expression of different alpha- and beta-tubulin genes, referred to as tubulin isotypes, and the generation of posttranslational modifications (PTMs) on alpha- and beta-tubulin. Tubulin PTMs include the well-known acetylation or phosphorylation, and others that have so far mostly been found on tubulin, detyrosination/tyrosination, polyglutamylation and polyglycylation. These PTMs might have evolved to specifically regulate tubulin and microtubule functions. Tubulin acetylation was discovered on K40 of flagellar alpha-tubulin in Chlamydomonas reinhardtii and is generally enriched on stable microtubules in cells. It is located on the microtubule lumenal surface. As a result of its localization at the inner face of microtubules, K40 acetylation might rather affect the binding of microtubule inner proteins, a poorly characterized family of proteins. Functional experiments in cells have further suggested that K40 acetylation regulates intracellular transport by regulating the traffic of kinesin motors probably by indirect mechanisms. Acetyltransferase alpha-Tat1 (or Mec-17) specifically acetylate alpha-tubulin K40. Acetylation of tubulin by alpha-Tat1 accumulates selectively in stable, long-lived microtubules thus explaining the link between this posttranslational modication and stable microtubules in cells. However, the direct cellular function of K40 acetylation on microtubules is still unclear. - Monoclonal Antibody. Detects K40 acetylation of alpha-tubulin; signal specifically increased by modification with tubulin acetyl transferase alpha-TAT1. Isotype: Mouse IgG1. Clone: TEU318. Applications: ICC, WB. Liquid. In PBS containing 10% glycerol and 0.02% sodium azide. Microtubules are key elements of the eukaryotic cytoskeleton that dynamically assemble from heterodimers of alpha- and beta-tubulin. Two different mechanisms can generate microtubule diversity: the expression of different alpha- and beta-tubulin genes, referred to as tubulin isotypes, and the generation of posttranslational modifications (PTMs) on alpha- and beta-tubulin. Tubulin PTMs include the well-known acetylation or phosphorylation, and others that have so far mostly been found on tubulin, detyrosination/tyrosination, polyglutamylation and polyglycylation. These PTMs might have evolved to specifically regulate tubulin and microtubule functions. Tubulin acetylation was discovered on K40 of flagellar alpha-tubulin in Chlamydomonas reinhardtii and is generally enriched on stable microtubules in cells. It is located on the microtubule lumenal surface. As a result of its localization at the inner face of microtubules, K40 acetylation might rather affect the binding of microtubule inner proteins, a poorly characterized family of proteins. Functional experiments in cells have further suggested that K40 acetylation regulates intracellular transport by regulating the traffic of kinesin motors probably by indirect mechanisms. Acetyltransferase alpha-Tat1 (or Mec-17) specifically acetylate alpha-tubulin K40. Acetylation of tubulin by alpha-Tat1 accumulates selectively in stable, long-lived microtubules thus explaining the link between this posttranslational modication and stable microtubules in cells. However, the direct cellular function of K40 acetylation on microtubules is still unclear.
  • Reactivity
    All Species
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203