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Antibodies
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Primary Antibodies
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Western Blot
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Western blotting at 1:200, low percentage PAGE gels a must, protein is quite large, proteinase inhibitors recommended or lower level fragments likely, membrane preparations and enhance signals. ELISA: 1:1000 Immunoprecipitation: 1:100 Immunocytochemistry, using 2% paraformaldehyde fixation, higher fixation (i.e. 4% PFA can be problematic) 1:100, light fixation 5-10' RT usually, permeabilize lightly with 0.1% triton X-100 as protein is intracellular. Flow cytometry: 1:500 Immunohistochemistry: 1:100, light f
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Mouse
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Monoclonal
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IgG
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