anti-Caspase-8 (mouse), mAb (1G12)

Catalog number: AG-20T-0137-C100
Brand: AdipoGen Life Sciences
Packing: 100 ug
Price: € 295.00
Expected delivery time: 7 days
Quantity:

Product specifications for - anti-Caspase-8 (mouse), mAb (1G12)

Overview: 
Product group: Antibodies
Category: Primary Antibodies
Application: ELISA; Flow Cytometry; ImmunoHistoChemistry; Western Blot
Species: Mouse
Host: Rat
Target: Casp8
Target description: caspase 8
Clonality: Monoclonal
Isotype: IgG1 Kappa
Properties: 
Purity: >95% (SDS-PAGE)
Datasheet: Datasheet
  Research Use Only
UNSPSC: 12352203
Concentration: 1 mg/ml
Form supplied: Liquid. In PBS containing 0.02% sodium azide.
Storage instructions: 2-¦C to 8-¦C, -20-¦C
Scientific information: 
Scientific info: Monoclonal Antibody. Recognizes the p20 subunit of mouse caspase-8. Detects bands of ~55kDa (full-length caspase-8) and ~18kDa (apoptosis-induced cleavage fragment) by Western blot. Does not cross-react with human caspase-8. Isotype: Rat IgG1kappa. Clone: 1G12. Applications: ELISA, FACS, IHC, WB. Liquid. In PBS containing 0.02% sodium azide. Procaspase-8 belongs to the family of caspases. Binding of FasL to Fas leads to formation of a receptor complex at the cellular membrane, which was named DISC. The DISC consists of oligomerized receptors, the DD-containing adaptor molecule FADD, procaspase-8, procaspase-10 and c-FLIP. The DISC structure provides a platform for the oligomerization of procaspase-8 that allows two procaspase-8 homodimers to be in the close proximity leading to the initial activation of procaspase-8. At the first cleavage step, the N-terminal p43/p41 and the C-terminal p30 cleavage products are generated. Importantly, these cleavage products already possess catalytic activity. At the second cleavage step, p43/p41 and p30 are processed to p10 and p20, respectively, which leads to the generation of the active caspase-8 heterotetramer (p20/p10)2. - Procaspase-8 belongs to the family of caspases. Binding of FasL to Fas leads to formation of a receptor complex at the cellular membrane, which was named DISC. The DISC consists of oligomerized receptors, the DD-containing adaptor molecule FADD, procaspase-8, procaspase-10 and c-FLIP. The DISC structure provides a platform for the oligomerization of procaspase-8 that allows two procaspase-8 homodimers to be in the close proximity leading to the initial activation of procaspase-8. At the first cleavage step, the N-terminal p43/p41 and the C-terminal p30 cleavage products are generated. Importantly, these cleavage products already possess catalytic activity. At the second cleavage step, p43/p41 and p30 are processed to p10 and p20, respectively, which leads to the generation of the active caspase-8 heterotetramer (p20/p10)2.
Clone ID: 1G12
Gene ID: 12370
Swiss prot ID: O89110
Safety information: 
MSDS: MSDS
Hazard information: Non-hazardous
Additional information: 
Synonyms: AG-20T-0137-C100; AdipoGen Life Sciences; CASP-8; caspase-8; Fas-linked ICE-like protease; FLICE; MACH; Mch5
Apaf-1 and caspase-9 do not act as tumor suppressors in myc-induced lymphomagenesis or mouse embryo fibroblast transformation: C.L. Scott, et al.; J. Cell Biol. 164, 89 (2004) Read more
Modifications and intracellular trafficking of FADD/MORT1 and caspase-8 after stimulation of T lymphocytes: L.A. O'Reilly, et al.; Cell Death Differ. 11, 724 (2004) Read more
Caspase-8 serves both apoptotic and nonapoptotic roles: T.B.Kang, et al.; J. Immunol. 73, 2976 (2004) Read more
Role of membrane sphingomyelin and ceramide in platform formation for Fas-mediated apoptosis: M. Miyaji, et al.; J. Exp. Med. 202, 249 (2005) Read more
Fatal Hepatitis Mediated by Tumor Necrosis Factor TNFalpha Requires Caspase-8 and Involves the BH3-Only Proteins Bid and Bim: T. Kaufmann, et al.; Immunity 30, 56 (2009) Read more
Catalytic activity of the caspase-8-FLIPL complex inhibits RIPK3-dependent necrosis: A. Oberst, et al.; Nature 471, 363 (2011) Read more
FLIP (Flice-like inhibitory protein) suppresses cytoplasmic double-stranded-RNA-induced apoptosis and NF-B and IRF3-mediated signaling: P. Handa, et al.; Cell Commun. Signal. 9, 16 (2011) Read more
XIAP restricts TNF- and RIP3-dependent cell death and inflammasome activation: M. Yabal, et al.; Cell Rep. 7, 1796 (2014) Read more