![Western blot using anti-Caspase-8 (mouse), mAb (3B10) (Prod. No. AG-20T-0138) detecting endogenous caspase-8 in various mouse cell line, but not in human cell lines. Western blot using anti-Caspase-8 (mouse), mAb (3B10) (Prod. No. AG-20T-0138) detecting endogenous caspase-8 in various mouse cell line, but not in human cell lines.](https://adipogen.com/pub/media/catalog/product/a/g/ag-20t-0138-wb_mouse-human_cell_lines.png)
Western blot using anti-Caspase-8 (mouse), mAb (3B10) (Prod. No. AG-20T-0138) detecting endogenous caspase-8 in various mouse cell line, but not in human cell lines.
anti-Caspase-8 (mouse), mAb (3B10)
![Research Use Only](static/images/certificates/ruo.jpg)
AG-20T-0138
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse
TargetCasp8
Overview
- SupplierAdipoGen Life Sciences
- Product Nameanti-Caspase-8 (mouse), mAb (3B10)
- Delivery Days Customer10
- Antibody SpecificityRecognizes the p20 subunit of mouse caspase-8. Detects bands of ~55kDa (full-length caspase-8) and ~18kDa (apoptosis-induced cleavage fragment) by Western blot. Does not cross-react with human caspase-8.
- ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID3B10
- Concentration1 mg/ml
- Estimated Purity>95%
- Gene ID12370
- Target nameCasp8
- Target descriptioncaspase 8
- Target synonymsCasp; CASP-8; caspase-8; Fas-linked ICE-like protease; FLI; FLICE; MAC; MACH; Mch5
- HostRat
- IsotypeIgG1
- Scientific DescriptionMonoclonal Antibody. Recognizes the p20 subunit of mouse caspase-8. Detects bands of ~55kDa (full-length caspase-8) and ~18kDa (apoptosis-induced cleavage fragment) by Western blot. Does not cross-react with human caspase-8. Isotype: Rat IgG1kappa. Clone: 3B10. Applications: ELISA, FACS, ICC, IHC, WB. Liquid. In PBS containing 0.02% sodium azide. Procaspase-8 belongs to the family of caspases. Binding of FasL to Fas leads to formation of a receptor complex at the cellular membrane, which was named DISC. The DISC consists of oligomerized receptors, the DD-containing adaptor molecule FADD, procaspase-8, procaspase-10 and c-FLIP. The DISC structure provides a platform for the oligomerization of procaspase-8 that allows two procaspase-8 homodimers to be in the close proximity leading to the initial activation of procaspase-8. At the first cleavage step, the N-terminal p43/p41 and the C-terminal p30 cleavage products are generated. Importantly, these cleavage products already possess catalytic activity. At the second cleavage step, p43/p41 and p30 are processed to p10 and p20, respectively, which leads to the generation of the active caspase-8 heterotetramer (p20/p10)2. - Procaspase-8 belongs to the family of caspases. Binding of FasL to Fas leads to formation of a receptor complex at the cellular membrane, which was named DISC. The DISC consists of oligomerized receptors, the DD-containing adaptor molecule FADD, procaspase-8, procaspase-10 and c-FLIP. The DISC structure provides a platform for the oligomerization of procaspase-8 that allows two procaspase-8 homodimers to be in the close proximity leading to the initial activation of procaspase-8. At the first cleavage step, the N-terminal p43/p41 and the C-terminal p30 cleavage products are generated. Importantly, these cleavage products already possess catalytic activity. At the second cleavage step, p43/p41 and p30 are processed to p10 and p20, respectively, which leads to the generation of the active caspase-8 heterotetramer (p20/p10)2.
- ReactivityMouse
- Storage Instruction2°C to 8°C,-20°C
- UNSPSC12352203