Western blot of 293T cells transfected (+) or untransfected (-) with a DNA construct encoding His-Tag Histone H3 (G34W) protein, using chimeric human anti-HisTag antibody Clone RMH01 at 0.2ug/mL, followed by a HRP conjugated anti-Human IgG secondary antibody.
Western blot of 293T cells transfected (+) or untransfected (-) with a DNA construct encoding His-Tag Histone H3 (G34W) protein, using chimeric human anti-HisTag antibody Clone RMH01 at 0.2ug/mL, followed by a HRP conjugated anti-Human IgG secondary antibody.
anti-His-Tag Chimeric Human, Rabbit Monoclonal (RMH01)
REV-54-1161-00
Overview
- SupplierRevMAb Biosciences
- Product Nameanti-His-Tag Chimeric Human, Rabbit Monoclonal (RMH01)
- Delivery Days Customer5
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDRMH01
- Concentration1 mg/ml
- HostHuman
- IsotypeIgG
- Scientific DescriptionHis-tag is a series of six to nine histidine residues fused to either the carboxyl or amino terminus of a recombinant protein. The small size of the His-tag, compared with other common epitope tags, makes it less likely to obstruct the target proteins structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions allows for the simple purification and detection of His-tagged proteins. His-tag antibodies provide a dependable method for the detection and purification of tagged target proteins without a protein-specific antibody or probe. - Recombinant Antibody. This antibody is a Human-Rabbit chimeric antibody, made by fusion of the antigen binding region (variable domains of the heavy and light chains, VH and VL) of rabbit anti-HisTag monoclonal antibody (RM146), with the constant domain of Human IgG1. The antibody is recognized only by an anti-Human IgG secondary antibody. Applications: WB, ELISA. Source: Rabbit. Liquid. 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide. His-tag is a series of six to nine histidine residues fused to either the carboxyl or amino terminus of a recombinant protein. The small size of the His-tag, compared with other common epitope tags, makes it less likely to obstruct the target proteins structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions allows for the simple purification and detection of His-tagged proteins. His-tag antibodies provide a dependable method for the detection and purification of tagged target proteins without a protein-specific antibody or probe.
- ReactivityAll Species
- Storage Instruction-20°C
- UNSPSC12352203