ELISA: 2 microg/ml dilution of recombinant human HMGB1, bovine HMGB1 and recombinant human HMGB2 proteins (left to right) were coated onto ELISA plate. ARG66714 anti-HMGB1 Neutralizing antibody [SQab20175] (low endotoxin) at 1:3500 dilution were used for detecting HMGB1.
![Flow Cytometry: HuH-7 cells (black) and HuH-7 shHMGB1 cells (red) stained with ARG66714 anti-HMGB1 Neutralizing antibody [SQab20175] (low endotoxin) at 1:500 dilution.](https://www.arigobio.com/files/product/22663/ARG66714_FACS_1.jpg "Flow Cytometry: HuH-7 cells (black) and HuH-7 shHMGB1 cells (red) stained with ARG66714 anti-HMGB1 Neutralizing antibody [SQab20175] (low endotoxin) at 1:500 dilution.")
![Neutralization assay: Cell culture medium from THP1 cells treated with 10 microg of isotype control mouse IgM, plus 2microg/ml of rhHMGB1 or not as control. THP1 cells treated with 2microg/ml of rhHMGB1 plus 0.1 microg, 1 microg or 10 microg of ARG66714 anti-HMGB1 Neutralizing antibody [SQab20175] (low endotoxin) (left to right) as assay groups. TNF alpha concentration was tested by ARG80120 Human TNF alpha ELISA Kit.](https://www.arigobio.com/files/product/22663/ARG66714_Neut_Human_200303.jpg "Neutralization assay: Cell culture medium from THP1 cells treated with 10 microg of isotype control mouse IgM, plus 2microg/ml of rhHMGB1 or not as control. THP1 cells treated with 2microg/ml of rhHMGB1 plus 0.1 microg, 1 microg or 10 microg of ARG66714 anti-HMGB1 Neutralizing antibody [SQab20175] (low endotoxin) (left to right) as assay groups. TNF alpha concentration was tested by ARG80120 Human TNF alpha ELISA Kit.")
![Neutralization assay: Cell culture medium from Raw264.7 cells treated with 10 microg of isotype control mouse IgM, plus 2 microg/ml of rhHMGB1 or not as control. Raw264.7 cells treated with 2 microg/ml of rmHMGB1 plus 0.1 microg, 1 microg or 10 microg of ARG66714 anti-HMGB1 Neutralizing antibody [SQab20175] (low endotoxin) (left to right) as assay groups. TNF alpha concentration was tested by ARG80206 Mouse TNF alpha ELISA Kit.](https://www.arigobio.com/files/product/22663/ARG66714_Neut_Mouse_200303.jpg "Neutralization assay: Cell culture medium from Raw264.7 cells treated with 10 microg of isotype control mouse IgM, plus 2 microg/ml of rhHMGB1 or not as control. Raw264.7 cells treated with 2 microg/ml of rmHMGB1 plus 0.1 microg, 1 microg or 10 microg of ARG66714 anti-HMGB1 Neutralizing antibody [SQab20175] (low endotoxin) (left to right) as assay groups. TNF alpha concentration was tested by ARG80206 Mouse TNF alpha ELISA Kit.")
![Western blot: 100 ng of HMGB1 and HMGB2 recombinant proteins stained with ARG66714 anti-HMGB1 Neutralizing antibody [SQab20175] (low endotoxin).](https://www.arigobio.com/files/product/22663/ARG66714_WB_1.jpg "Western blot: 100 ng of HMGB1 and HMGB2 recombinant proteins stained with ARG66714 anti-HMGB1 Neutralizing antibody [SQab20175] (low endotoxin).")
ELISA: 2 microg/ml dilution of recombinant human HMGB1, bovine HMGB1 and recombinant human HMGB2 proteins (left to right) were coated onto ELISA plate. ARG66714 anti-HMGB1 Neutralizing antibody [SQab20175] (low endotoxin) at 1:3500 dilution were used for detecting HMGB1.
anti-HMGB1 Neutralizing antibody [SQab20175] (low endotoxin)
ARG66714
ApplicationsFlow Cytometry, Western Blot, ELISA, Neutralisation/Blocking
Product group Antibodies
ReactivityHuman
TargetHMGB1
Overview
- SupplierArigo Biolaboratories
- Product Nameanti-HMGB1 Neutralizing antibody [SQab20175] (low endotoxin)
- Delivery Days Customer23
- ApplicationsFlow Cytometry, Western Blot, ELISA, Neutralisation/Blocking
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDSQab20175
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID3146
- Target nameHMGB1
- Target descriptionhigh mobility group box 1
- Target synonymsAmphoterin; high mobility group protein B1; high-mobility group (nonhistone chromosomal) protein 1; HMG1; HMG-1; HMG3; SBP-1; Sulfoglucuronyl carbohydrate binding protein
- HostMouse
- IsotypeIgM
- Scientific DescriptionHMGB1 is a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]
- ReactivityHuman
- Storage Instruction2°C to 8°C or -20°C
- UNSPSC12352203