Antibodies
|
Primary Antibodies
|
Flow Cytometry
|
Flow Cytometry: 1-3 ug LM609 per 0.5 x 10e6 cells in 100uL (Smith, 1996). Immunofluorescence: 5-10 ug/mL for staining of bFGF-treated 6 mm cryosections of chick chorioallantoic membrane fixed with acetone (Brooks, 1994). LM609 is also effective on 4% paraformaldehyde fixed, frozen tissues and cells. However it is not effective for immunohistochemical staining of paraffin-embedded tissue sections. Optimal working dilutions and protocols must be determined by end user.
|
Mouse
|
ITGAV
|
integrin, alpha V
|
Monoclonal
|