Gel: 15%+12% SDS-PAGELane1: Human liver Cancer tissue lysate Lane2: Human leiomyosarcoma tissue lysate. Lysates: 40 µg per lane. Primary antibody: 1/250 dilution. Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution. Exposure time: 10 seconds