Western blot analysis of extracts of Jurkat cell line, using Anti-PTK2 (phospho Y925) Antibody (AP0599) 1:1000 dilution.
Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution.
Lysates / proteins: 25µg per lane.
Blocking buffer: 3% BSA.
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