Anti-SGLT-1 (Sodium Glucose Co-transporter-1) Antibody

Catalog number: 07-1417
Brand: Merck
Packing: 100 ul
Price: On Request
Expected delivery time: 7 days

Product specifications for - Anti-SGLT-1 (Sodium Glucose Co-transporter-1) Antibody

Product group: Antibodies
Category: Primary Antibodies
Application: Immunohistochemisty
Application note: Immunohistochemisty (paraffin): Anti-SGLT-1 staining on human kidney, tissue pretreated with citrate buffer, pH 6.0. A previous lot of this antibody was used at 1 ug/mL, using IHC-Select detection with HRP-DAB. Immunoreactivity is detected in the brush boarder of proximal convoluted tubule.ELISA: 1:10,000 - 1:50,000 dilution of a previous lot, using 50-100 ng of control peptide/well.Optimal working dilutions must be determined by end user.Western Blot: A previous lot of this antibody was used on multiple ly
Host: Rabbit
Clonality: Polyclonal
  Research Use Only
UNSPSC: 12352203
Storage instructions: Stable for 1 year at -20-¦C from date of receipt in undiluted aliquots.Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20-¦C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Scientific information: 
Scientific info: The kidneys play a major role in the regulation of blood glucose. Although kidney nephrons indiscriminatly filter about 180g of glucose per day from the blood, most is reabsorbed actively in the proximal convoluted tubule by a Na+/Gl co-transport (ratio 2:1) mechanism (SGLT). Typically, glucose is first reabsorbed by these transporters, which reside within the brush-border epithelia of the PCT cells. The glucose is then transported out of the cell across the basolateral membranes by a facilitated diffusion sugar transporter (GLUTs). At least three members of SGLT's (SGLT1-3) have been cloned and characterized from various species. SGLT1-3 genes code for protein of approximately 659-672 residues, with both N and C termini predicted to be extracellular. There is approximately 60-70% homology between the 3 forms. SGLT1 does not discriminate a-MDG, glucose and galactose, while SGLT2 and 3 do not transport D-galactose efficiently. SGLT1 is an integral membrane protein expressed in proximal convoluted tubule Sq1 segments of the kidney (outer renal medulla) and in the intestine.
Additional information: 
Synonyms: 07-1417; Merck (Millipore)