Tissue/cell:MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Estrogen Receptor alpha) polyclonal Antibody, Unconjugated (bs-0122R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Tissue/cell:MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Estrogen Receptor alpha) polyclonal Antibody, Unconjugated (bs-0122R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Estrogen Receptor alpha Antibody
BS-0122R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityBovine, Canine, Human, Mouse, Rabbit, Rat
Overview
- SupplierBioss Antibodies
- Product NameEstrogen Receptor alpha Antibody
- Delivery Days Customer16
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- HostRabbit
- IsotypeIgG
- ReactivityBovine, Canine, Human, Mouse, Rabbit, Rat
- Storage Instruction-20°C
- UNSPSC12352203