U2OS cells were stained with the Bioss antibody against H3K36me1 (cat. No. bs-53131R) and with DAPI. Cells were fixed with 4% formaldehyde for 20’ and blocked with PBS/TX-100 containing 5% normal goat serum. Figure A: cells were immunofluorescently labeled with the H3K36me1 antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa568 or with DAPI (right), which specifically labels DNA. Figure B, C, D, and E: staining of the cells with the H3K36me1 antibody after incubation of the antibody with 2 ng/μl blocking peptide containing the unmodified and the mono-, di- and trimethylated H3K36, respectively.
H3K36me1 Polyclonal Antibody
BS-53131R
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
Overview
- SupplierBioss Antibodies
- Product NameH3K36me1 Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ELISA
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), IF(), dot-blot()
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.95 ug/ul
- ConjugateUnconjugated
- HostRabbit
- ReactivityHuman
- Storage Instruction-20°C
- UNSPSC12352203