ChIP assays were performed using HeLa cells, the H3K4me3 antibody (bs-53034R) and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 1 million cells. A titration consisting of 1, 2, 5 and 10 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoter of the active genes GAPDH and EIF4A2, used as positive controls, and for exon 2 of the inactive myoglobin (MB) gene and the Sat2 satellite repeat, used as negative controls. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that trimethylation of K4 at histone H3 is associated with the promoters of active genes
H3K4me3 Polyclonal Antibody
BS-53034R
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
Overview
- SupplierBioss Antibodies
- Product NameH3K4me3 Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- Applications SupplierWB(1:300-5000), IF(ICC)(1:50-200), IF(), dot-blot()
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- HostRabbit
- ReactivityHuman
- Storage Instruction-20°C
- UNSPSC12352203