HeLa cells were stained with the Bioss antibody against H3K4me3 (Cat. No. bs-53103R) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H3K4me3 antibody (left) diluted 1:100 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
H3K4me3 Polyclonal Antibody
BS-53103R
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation
Product group Antibodies
ReactivityHuman, Mouse, Plant, Other Species
Overview
- SupplierBioss Antibodies
- Product NameH3K4me3 Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation
- Applications SupplierWB(1:300-5000), IF(), dot-blot(), ChIP-seq()
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration2 ug/ul
- ConjugateUnconjugated
- HostRabbit
- ReactivityHuman, Mouse, Plant, Other Species
- Storage Instruction-20°C
- UNSPSC12352203