GTX30647 WB Image
HIF1 alpha antibody - Orthogonal Validated
GTX30647
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityGoat, Hamster, Human, Monkey, Mouse, Primate, Rabbit, Rat
Overview
- SupplierGeneTex
- Product NameHIF1 alpha antibody - Orthogonal Validated
- Delivery Days Customer10
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- HostRabbit
- IsotypeIgG
- Scientific DescriptionFunctions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions activates the transcription of over 40 genes, including, erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5-[AG]CGTG-3 within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP.
- ReactivityGoat, Hamster, Human, Monkey, Mouse, Primate, Rabbit, Rat
- Storage Instruction2°C to 8°C
- UNSPSC12352203
References
- Akt/hypoxia-inducible factor-1alpha signaling deficiency compromises skin wound healing in a type 1 diabetes mouse model. Jing L et al., 2015 Jun, Exp Ther MedRead more
- Endothelial Sirtuin 3 Dictates Glucose Transport to Cardiomyocyte and Sensitizes Pressure Overload-Induced Heart Failure. Zeng H et al., 2020 Jun 2, J Am Heart AssocRead more
- Hypoxia-mediated control of HIF/ARNT machinery in epidermal keratinocytes. Weir L et al., 2011 Jan, Biochim Biophys ActaRead more