Paraformaldehyde-fixed, paraffin embedded rat bladder tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-p53 (FL-393) Polyclonal Antibody, Unconjugated (bs-8687R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining
p53 (FL-393) Polyclonal Antibody
BS-8687R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Equine, Human, Mouse, Porcine, Rat, Sheep
Overview
- SupplierBioss Antibodies
- Product Namep53 (FL-393) Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200), ICC(1:100-500)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- HostRabbit
- IsotypeIgG
- ReactivityBovine, Canine, Equine, Human, Mouse, Porcine, Rat, Sheep
- Storage Instruction-20°C
- UNSPSC12352203
References
- Rotenone and 3-bromopyruvate toxicity impacts electrical and structural cardiac remodeling in rats. Zhan C et al., 2020 Jan, Toxicol LettRead more
- Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable. Zhang W et al., 2018 Mar, ChemosphereRead more
- Effects of microRNA-211 on proliferation and apoptosis of lens epithelial cells by targeting SIRT1 gene in diabetic cataract mice. Zeng K et al., 2017 Aug 31, Biosci RepRead more
- Effects of irreversible electroporation on cervical cancer cell lines in vitro. Qin Q et al., 2016 Sep, Mol Med RepRead more