HL-60 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with SirT1 (Ser27) Polyclonal Antibody(bs-3416R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
SirT1 (Ser27) Polyclonal Antibody
BS-3416R
ApplicationsFlow Cytometry, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
Overview
- SupplierBioss Antibodies
- Product NameSirT1 (Ser27) Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsFlow Cytometry, Western Blot, ELISA
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- HostRabbit
- IsotypeIgG
- Modification TypePhosphorylated
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C
- UNSPSC12352203