Paraformaldehyde-fixed, paraffin embedded rat spleen tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (BSA or normal goat serum) at 37°C for 20min; Antibody incubation with TLR4 Polyclonal Antibody, Unconjugated bs-20595R at 1:200 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat spleen tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (BSA or normal goat serum) at 37°C for 20min; Antibody incubation with TLR4 Polyclonal Antibody, Unconjugated bs-20595R at 1:200 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
TLR4 Polyclonal Antibody
BS-20595R
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Human, Mouse, Porcine, Rat, Sheep
TargetTLR4
Overview
- SupplierBioss Antibodies
- Product NameTLR4 Polyclonal Antibody
- Delivery Days Customer16
- Antibody SpecificityMouse TLR4 has 75% homology to the antibody immunogen sequence
- ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID7099
- Target nameTLR4
- Target descriptiontoll like receptor 4
- Target synonymsARMD10; CD284; homolog of Drosophila toll; hToll; TLR-4; TOLL; toll like receptor 4 protein; toll-like receptor 4
- HostRabbit
- IsotypeIgG
- Protein IDO00206
- Protein NameToll-like receptor 4
- ReactivityBovine, Human, Mouse, Porcine, Rat, Sheep
- Storage Instruction-20°C
- UNSPSC12352203