Paraformaldehyde-fixed, paraffin embedded mouse testis tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Aph-1b Polyclonal Antibody, Unconjugated (bs-11640R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining
Aph-1b Polyclonal Antibody
BS-11640R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Human, Mouse, Porcine, Rat, Sheep
TargetAPH1B
Overview
- SupplierBioss Antibodies
- Product NameAph-1b Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID83464
- Target nameAPH1B
- Target descriptionaph-1 homolog B, gamma-secretase subunit
- Target synonymsanterior pharynx defective 1 homolog B; APH-1B; APH1B gamma secretase subunit; aph-1beta; gamma-secretase subunit APH-1B; PRO1328; PSFL; TAAV688
- HostRabbit
- IsotypeIgG
- Protein IDQ8WW43
- Protein NameGamma-secretase subunit APH-1B
- ReactivityBovine, Canine, Human, Mouse, Porcine, Rat, Sheep
- Storage Instruction-20°C
- UNSPSC12352203