
Apolipoprotein A1 antibody detects Apolipoprotein A1 protein at Golgi apparatus by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Apolipoprotein A1 stained by Apolipoprotein A1 antibody (GTX112692) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10μm.
Apolipoprotein A1 antibody
GTX112692
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetAPOA1
Overview
- SupplierGeneTex
- Product NameApolipoprotein A1 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.58 mg/ml
- ConjugateUnconjugated
- Gene ID335
- Target nameAPOA1
- Target descriptionapolipoprotein A1
- Target synonymsAMYLD3, HPALP2, apo(a), apolipoprotein A-I, apo-AI, epididymis secretory sperm binding protein
- HostRabbit
- IsotypeIgG
- Protein IDP02647
- Protein NameApolipoprotein A-I
- Scientific DescriptionThis gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The protein promotes cholesterol efflux from tissues to the liver for excretion, and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Konaka H, Kato Y, Hirano T, et al. Secretion of mitochondrial DNA via exosomes promotes inflammation in Behçet's syndrome. EMBO J. 2023,42(20):e112573. doi: 10.15252/embj.2022112573Read this paper
- Karimi N, Dalirfardouei R, Dias T, et al. Tetraspanins distinguish separate extracellular vesicle subpopulations in human serum and plasma - Contributions of platelet extracellular vesicles in plasma samples. J Extracell Vesicles. 2022,11(5):e12213. doi: 10.1002/jev2.12213Read this paper
- Gomes FG, Andrade AC, Wolf M, et al. Synergy of Human Platelet-Derived Extracellular Vesicles with Secretome Proteins Promotes Regenerative Functions. Biomedicines. 2022,10(2). doi: 10.3390/biomedicines10020238Read this paper
- Ekström K, Crescitelli R, Pétursson HI, et al. Characterization of surface markers on extracellular vesicles isolated from lymphatic exudate from patients with breast cancer. BMC Cancer. 2022,22(1):50. doi: 10.1186/s12885-021-08870-wRead this paper
- Sawada N, Obama T, Koba S, et al. Circulating oxidized LDL, increased in patients with acute myocardial infarction, is accompanied by heavily modified HDL. J Lipid Res. 2020,61(6):816-829. doi: 10.1194/jlr.RA119000312Read this paper
- Onódi Z, Pelyhe C, Terézia Nagy C, et al. Isolation of High-Purity Extracellular Vesicles by the Combination of Iodixanol Density Gradient Ultracentrifugation and Bind-Elute Chromatography From Blood Plasma. Front Physiol. 2018,9:1479. doi: 10.3389/fphys.2018.01479Read this paper











![Mouse plasma and Rat plasma (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Apolipoprotein A1 antibody [HL1713] (GTX637312) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637312/GTX637312_T-44774_20220819_WB_M_R_22082402_697.webp)

![WB analysis of HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY APOA1 cDNA (2) using GTX83043 Apolipoprotein A1 antibody [5F4F5].](https://www.genetex.com/upload/website/prouct_img/normal/GTX83043/GTX83043_20170912_WB_w_23061322_497.webp)


