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Apolipoprotein A1 antibody

GTX112692
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetAPOA1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Apolipoprotein A1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.58 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID335
  • Target name
    APOA1
  • Target description
    apolipoprotein A1
  • Target synonyms
    apo(a); apo-AI; apolipoprotein A-I; epididymis secretory sperm binding protein; HPALP2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP02647
  • Protein Name
    Apolipoprotein A-I
  • Scientific Description
    This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The protein promotes cholesterol efflux from tissues to the liver for excretion, and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Secretion of mitochondrial DNA via exosomes promotes inflammation in Behcets syndrome.
    Read more
  • Tetraspanins distinguish separate extracellular vesicle subpopulations in human serum and plasma - Contributions of platelet extracellular vesicles in plasma samples. Karimi N et al., 2022 May, J Extracell Vesicles
    Read more
  • Synergy of Human Platelet-Derived Extracellular Vesicles with Secretome Proteins Promotes Regenerative Functions. Gomes FG et al., 2022 Jan 23, Biomedicines
    Read more
  • Characterization of surface markers on extracellular vesicles isolated from lymphatic exudate from patients with breast cancer. Ekstroem K et al., 2022 Jan 10, BMC Cancer
    Read more
  • Circulating oxidized LDL, increased in patients with acute myocardial infarction, is accompanied by heavily modified HDL. Sawada N et al., 2020 Jun, J Lipid Res
    Read more
  • Isolation of High-Purity Extracellular Vesicles by the Combination of Iodixanol Density Gradient Ultracentrifugation and Bind-Elute Chromatography From Blood Plasma. Onodi Z et al., 2018, Front Physiol
    Read more

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Figure 1. Western blot analysis of APOA1 using anti-APOA1 antibody (PB9916). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APOA1 antigen affinity purified polyclonal antibody (Catalog # PB9916) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APOA1 at approximately 26 kDa. The expected band size for APOA1 is at 31 kDa.
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