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Annexin V-FITC/PI Apoptosis Assay Kit | ARG80927 | Arigo Biolaboratories available at Bio-Connect

Annexin V-FITC/PI Apoptosis Assa... | ARG80927 | Arigo Biolaboratories

Annexin V-FITC/PI Apoptosis Assay Kit

Assays

Annexins are a family of calcium-dependent phospholipid-binding proteins. They are abundant in eukaryotic organisms belonging to a family of ubiquitous cytoplasmic proteins involved in signal transduction. All annexins have been shown to have a putative binding site for protein kinase C (PKC) but only annexin V would possess a potential pseudo- substrate site. Thus annexin V seems to modulate the activity of some PKCs on their substrates.AnnexinV was found to play a major role in matrix vesicle-initiated cartilage calcification as a collage-regulated calcium channel. Annexin V binds to procoagulant phospholipids (Vascular anticoagulant alpha) with high affinity.Annexin Vs preferential binding partner is phosphatidylserine (PS). PS is predominantly located in membrane leaflets, which face the cytosol. However, recent findings show that each cell type has the molecular machinery to expose PS at its cell surface. This machinery is activated during the execution of apoptosis. Once PS is exposed at the cell surface it exhibits procoagulant and proinflammatory activities. Annexin V will bind to the PS-exposing apoptotic cell and can inhibit the procoagulant and proinflammatory activities of the dying cell.

ARG81299 | Cell Cycle / Apoptosis Analysis Kit | Arigo Biolaboratories available at Bio-Connect | Order online now!

Cell Cycle / Apoptosis Analysis Kit | ARG81299 | Arigo Biolaboratories

Cell Cycle / Apoptosis Analysis Kit

The content of DNA is changed with the process of cell cycle. And the DNA can be stained by fluorescent dye to measure its intensity by flow cytometry to monitor the cell cycle distribution in G1, S, GS/M phase and as well as apoptosis and aneuploidy cells with signals at sub-G1 or super G2 region. Propidium iodide (or PI) is a fluorescent intercalating agent and it binds to DNA by intercalating between the bases with little or no sequence preference. Therefore PI is used widely as a DNA dye in flow cytometry to evaluate cell viability or DNA content in cell cycle analysis, or in microscopy to visualize the nucleus and other DNA-containing organelles. However, PI also binds to RNA, and it interference with the DNA measurement in the assay. In this Cell Cycle / Apoptosis Analysis Kit we provide an RNase to degrade RNA before PI staining to eliminate the interference by RNA and the kit can be used to monitor cell cycle progression, proliferation and apoptosis by flow cytometry.

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Apoptosis

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